Special nutrition in mouse developmental oocytes

Proteome of mouse oocytes at different developmental stages.

The mammalian oocyte possesses powerful reprogramming factors, which can reprogram terminally differentiated germ cells (sperm) or somatic cells within a few cell cycles. Although it has been suggested that use of oocyte-derived transcripts may enhance the generation of induced pluripotent stem cells, the reprogramming factors in oocytes are undetermined, and even the identified proteins compos...

The Effect of Progesterone on the In vitro Maturation and Developmental Competence of Mouse Germinal Vesicle Oocytes

a:4:{s:10:"Background";s:206:"The aim of the present study was to investigate the role of progesterone on the developmental competence of cumulus-oocyte complexes (COCs) and cumulus-denuded oocytes (CDOs) at germinal vesicle (GV) stage.";s:19:"MaterialsAndMethods";s:485:"GV oocytes of pregnant mare's serum gonadotropin (PMSG)-primed prepubertal mice were divided into two groups: CDOs and COCs. ...

O-3: Effect of Melatonin Treatment on Developmental Potential of Somatic Cell Nuclear- Transferred Mouse Oocytes In Vitro

Background Melatonin (N-acetyl-5- methoxytryptamine) is mainly synthesized and secreted in the pineal gland, ovary, testes, bone marrow, retina and lens in mammalian species. It is involved in the detoxification of ROS and protects embryos from oxidative damage. Melatonin acts as a potential free radical scavenger, including peroxyl radical and hydroxyl radical. In addition, it can stimulate th...

Mouse Oocytes and Embryos Cryotop-vitrification Using Low Concentrated Solutions: Effects on Meiotic Spindle, Genetic Material Array and Developmental Ability

Dcp1-bodies in mouse oocytes.

Processing bodies (P-bodies) are cytoplasmic granules involved in the storage and degradation of mRNAs. In somatic cells, their formation involves miRNA-mediated mRNA silencing. Many P-body protein components are also found in germ cell granules, such as in mammalian spermatocytes. In fully grown mammalian oocytes, where changes in gene expression depend entirely on translational control, RNA g...